Identification of the Elusive Mammalian Enzyme Phosphatidylcholine-Specific Phospholipase C PRINCIPAL INVESTIGATOR:

Award Number : W 81 XWH - 13 - 1 - 0152 TITLE : Identification of the Elusive Mammalian Enzyme Phosphatidylcholine - Specific Phospholipase

Isolation and properties of a phosphatidylcholine-specific phospholipase C from bull seminal plasma.

A phospholipase C which hydrolyzes [14C]phosphatidylcholine has been purified 1782-fold from 70% ammonium sulfate extract of bull seminal plasma. Purification steps included acid precipitation, chromatography on DEAE-Sephacel, concanavalin A, octyl-Sepharose 4B and Ultrogel AcA 34. The final step provided homogeneous phospholipase C as determined by polyacrylamide gel electrophoresis. The enzym...

STUDIES ON PHOSPHOLIPASE C FROM MELIA AZADIRACHTA SEEDS

The activity of phospholipase C in crude enzymatic preparation of Melia azadiracht seeds (Neem seeds) was studied by the use of lecithin as a substrate in aqueous medium. The enzyme activity was found optimum at pH 2.5 and temperature 35?C. The phospholipase C was found heat labile, being inactivated 88% within 10 minutes at 90°C

Didecanoyl phosphatidylcholine is a superior substrate for assaying mammalian phospholipase D.

Phospholipase D (PLD) activity in crude or solubilized membranes from mammalian tissues is difficult to detect with the current assay techniques, unless a high radioactive concentration of substrate and/or long incubation times are employed. Generally, the enzyme has to be extracted and partially purified on one column before easy detection of activity. Furthermore, PLD activity in cultured cel...

The Phospholipid-Dependence of Uridine

Specific degradation of the phospholipid membrane of guinea-pig liver microsomal fraction with phospholipase A inactivated glucuronyltransferase. The inactivation was reversed by phosphatidylcholine and mixed microsomal phospholipid micelles at concentrations similar to those present in intact microsomal preparations. The other commonly occurring phospholipids did not reactivate phospholipase A...